Density gradient centrifugation and swim-up for ICSI: useful, unsafe, or just unsuitable?
نویسندگان
چکیده
منابع مشابه
Comparative proteomic analysis of spermatozoa isolated by swim-up or density gradient centrifugation
BACKGROUND Reports about the morphologic and functional characteristics of spermatozoa prepared by density gradient centrifugation (DC) or swim-up (SU) have produced discordant results. We have performed a proteomic comparison of cells prepared by DC and SU providing a molecular insight into the differences between these two methods of sperm cell isolation. METHODS Protein maps were obtained ...
متن کاملComparison of in vitro fertilization outcomes in ICSI cycles after human sperm preparation by density gradient centrifugation and direct micro swim-up without centrifugation
OBJECTIVE The aim of this study was to evaluate the efficacy of a non-expensive, easy and fast technique (direct micro swim-up) for sperm preparation in intracytoplasmic sperm injection (ICSI) treatments without the use of centrifuge. METHODS We carried out a multicentric study in which a total of 140 ICSI-cycles were included. Sibling oocytes were divided into two groups according to semen p...
متن کاملCombination of density gradient centrifugation and swim-up methods effectively decreases morphologically abnormal sperms
Density gradient centrifugation (DGC) and swim-up techniques have been reported for semen preparation in assisted reproductive techniques in humans. We investigated whether semen preparation using a combination of DGC and swim-up techniques could effectively decrease morphologically abnormal human sperms at the ultrastructural level. Semen samples were obtained from 16 infertile males and fract...
متن کاملSperm DNA fragmentation and sex chromosome aneuploidy after swim-up versus density gradient centrifugation
Objective The aim of this study was to compare the efficacy of swim-up and density gradient centrifugation (DGC) for reducing the amount of sperm with fragmented DNA, sex chromosome aneuploidy, and abnormal chromatin structure. Methods Semen samples were obtained from 18 healthy male partners who attended infertility clinics for infertility investigations and were processed with swim-up and D...
متن کاملSemen preparation methods and sperm telomere length: density gradient centrifugation versus the swim up procedure
Previous studies have shown that both density gradient centrifugation (DGC) and swim up (SU) procedures can select spermatozoa with longer telomeres for assisted reproduction techniques (ART). However, it is unknown which approach is more effective. The aim of the present study was to compare the effects of these two methods on sperm telomere length (STL). A total of 150 normozoospermic subject...
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ژورنال
عنوان ژورنال: Journal of Assisted Reproduction and Genetics
سال: 2019
ISSN: 1058-0468,1573-7330
DOI: 10.1007/s10815-019-01602-x